US4103001A - Buffered phenol-glutaraldehyde sterilizing

Buffered Phenol: 1. Thaw liquefied phenol at 25-37°C. Usually we process 4 tubes of phenol at a time. Preparation of Buffer Saturated Phenol for DNA Extraction Place the bottle of phenol in the 65°C water bath to melt the crystals. 3. Add 8-hydroxyquinoline to a final concentration of 0.1 % w/v to the phenol. Mix to dissolve the 8-hydroxyquinoline. Phenol, Buffered Saturated, pH 8.0 | | P-188A | Spectrum Phenol, Buffered Saturated, pH 8.0 - Spectrum solutions utilize the highest quality raw materials appropriate to your product. Most raw materials meet or exceed the specifications established by the American Chemical Society. Where appropriate, many finished products are traceable to NIST Standard Reference Materials.

Acid guanidinium thiocyanate-phenol-chloroform extraction

(Note: Prepared buffered phenol solution should be kept at -20ºC in 40 mL aliquots in 50 mL polypropylene tubes). 6. Phenol extraction buffer: Add equal volume of extraction buffer (plus phosphatase and protease inhibitors solution from Step 4 ) and buffered phenol ( from Step 3 ) in a 15 mL Falcon tube and mix before use. Phenol - an overview | ScienceDirect Topics Phenol reagents are usually prepared by saturating the phenol with aqueous buffer and then adding an equal volume of chloroform. The addition of chloroform stabilizes the phenol, imparts a greater density to this organic extracting material, improves the efficiency of deproteinization of the sample, and also facilitates removal of lipids from 4-Nitrophenol - Wikipedia

What is Phenol? Medical Uses, Health Benefits, and Risks

Phenol - CSH Protocols To the melted phenol, add an equal volume of buffer (usually 0.5 M Tris-Cl [pH 8.0] at room temperature). Stir the mixture on a magnetic stirrer for 15 minutes. Turn off the stirrer, and when the two phases have separated, aspirate as much as possible of the upper (aqueous) phase using a glass pipette attached to a vacuum line equipped with US4103001A - Buffered phenol-glutaraldehyde sterilizing